Figure 2
Substrate selectivity of R306465. Human A2780 ovarian carcinoma cells were incubated with the indicated concentrations of R306465, vorinostat, panobinostat, MS-275 or Trichostatin A (TSA) for 24 h. Total cell lysates were prepared and analysed by SDS–PAGE. Levels of acetylated H3 and tubulin, and of total levels of H3 and tubulin and of p21waf1, cip1 and Hsp70 and c-raf protein, were detected using rabbit polyclonal and mouse monoclonal antibodies, followed by ECL detection as indicated in the Methods section. To control for equal loading, blots were stripped and re-probed with antibodies against actin and the nuclear protein Lamin B1. A representative experiment out of three is shown.
