Figure 1
(A) DNA in agarose gel after TMPRSS2:ERG-specific RT–PCR of RNA from primary prostate cancer tissue. Shown are PrR amplified products, from a few representative cases, resolved by electrophoresis on a 2% agarose gels. Primer sets producing an expected size of 125 bp poducts are shown. (B) Schematic diagram and electropherograms of TMPRSS2:ERG-specific RT–PCR amplified products. Diagramed are TMPRSS2 and ERG variant 2 (Genbank NM_00449) gene exons and fusion transcripts, with deleted exons in hatched colour, retained exons in solid colour, and the fusion junction boxed. Shown is a fusion junction for one of the cases, after electrophoresed DNA in bands (Figure 1A) was excised, purified and sequenced using an ABI 3700 (Applied Biosystems) automated DNA sequencer.
