Figure 1 | British Journal of Cancer

Figure 1

From: Alternative splicing variant of the hypoxia marker carbonic anhydrase IX expressed independently of hypoxia and tumour phenotype

Figure 1

Identification and predicted structure of the human splicing variant of CA IX. (A) Schematic illustration of the genomic structure of the human CA9 gene (GenBank no. Z54349). Positions of primers are indicated by arrows. Exons excluded by alternative splicing are in dark grey colour. (B) RT–PCR analysis of CA9 in the human stomach, small intestine, and colon using h1S-h6A primers that do not discriminate between the splicing variants. (C) Amplification of both FL and AS transcripts in the human tissues using h6S-h11A primers. (D) Comparison of amino acid sequences deduced from the human FL and AS CA9 cDNAs. Signal peptide (SP) is written in italic, proteoglycan-like domain (PG) is in bold, carbonic anhydrase domain (CA) is on a grey background, and the transmembrane region (TM) is written by white letters on a black background. Dashed lines represent amino acid residues deleted in AS. Histidines that bind a catalytic zinc and cysteines involved in formation of S-S bonds are placed on a dark background (E) Predicted structure of the human FL and AS CA IX proteins.

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