Figure 2 | British Journal of Cancer

Figure 2

From: Alternative splicing variant of the hypoxia marker carbonic anhydrase IX expressed independently of hypoxia and tumour phenotype

Figure 2

Expression of the AS CA IX variant in human tumour cell lines and in human tissues. RT–PCR analysis of human AS CA9 using the primers designed for individual amplification of the splicing variants, namely h7S-h8A for FL and h7S-h10/7A for AS (see Figure 3). β-actin was used as a standard. SmartLadder was included in the right side of the gels. The cDNAs were isolated (A) from the cells exposed to normoxia (N) and hypoxia (H) for 48 h, (B) from the cells incubated at low and high density for 72 h, and (C) from normal and tumour human tissues. The results indicate that the AS expression is steady and does not depend on hypoxia, density, and tumour phenotype.

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