Figure 3 | British Journal of Cancer

Figure 3

From: Alternative splicing variant of the hypoxia marker carbonic anhydrase IX expressed independently of hypoxia and tumour phenotype

Figure 3

Localisation of the human AS CA IX. CA IX-negative MDCK cells and HeLa cells with natural hypoxia-induced expression of FL CA IX were permanently transfected with AS CA9 cDNA in pSG5C plasmid. (A) Immunofluorescence analysis of the AS-transfected (AS), FL-transfected (FL), and control cells (mock) was performed using M75 MAb recognising both AS and FL proteins. (B) Immunoblotting analysis of the protein extracts from HeLa-AS and control HeLa cells plated in sparse (S), medium (M), and dense (D) cultures, allowed to adhere for 24 h and then incubated for 24 h in normoxia and hypoxia, respectively. Asterisk on the CA IX blot indicates the position of 50 K Mw marker, α-tubulin was used as a loading control. (C) The AS CA IX variant was also detected with M75 MAb in culture medium of AS-transfected cells.

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