Figure 2
Analysis of CpG island methylation status of the promoter of SOX9 by bisulphite genomic sequencing in bladder human cancer cell lines (n=11; including nonmuscle invasive, invasive, metastatic transitional and squamous cells). The upper part indicates the nucleotide sequences of the CpG island region analysed by bisulphite sequencing, sequencing primers highlighted in yellow and red and the area amplified in the chromatograms in blue. The mid-section shows a schematic depiction of the SOX9 CpG islands around the transcription start sites. CpG dinucleotides are represented in squares. The presence of ‘Cs’ in the dinucleotide CpG reflects methylated cytosines (black squares), while the presence of ‘Ts’ in the dinucleotide CpG reflects unmethylated cytosines (white squares). Cell lines with black squares indicate the presence of methylation confirmed in at least two of the clones that were sequenced for each of the cell lines under analyses. The bottom part displays representative examples of the chromatograms obtained by bisulphite genomic sequencing of human cancer cell lines (a magnified boxed fragment is displayed). Normal lymphocytes (NL) were used as a negative sequencing control.
