Table 2 EROD activity of MCF-7 cells

From: CYP1B1 expression is induced by docetaxel: effect on cell viability and drug resistance

 

EROD activity (picomoles resorufin per mg total protein per min)

MCF-7

ND

MCF-7 Txt

0.011±0.001

  1. EROD=ethoxyresorufin-O-deethylase; ND=not detectable.
  2. After addition of 7-ethoxy resorufin, cells were incubated at 37°C for 40 min. Samples were removed and spun down, and the amount of resorufin present in the supernatant determined by fluorescence (530 nm excitation; 590 nm emission). Results represent the average of five independent experiments. Activity is expressed as picomoles resorufin per mg of total protein per min.