Figure 1
From: Elimination of wild-type P53 mRNA in glioblastomas showing heterozygous mutations of P53
Molecular analyses of glioblastomas. (A) example of LOH analysis showing a minimal contamination of the tumour sample with the normal cells (case 4; only a trace of the lost allele is observed in the tumour sample). The lost allele is marked with an arrow. (B, C) P53-sequencing results. The mutated nucleotide (p53 Exon 8; codon 273; CGT>TGT; Arg>Cys) is marked with arrows. (B) genomic DNA sequencing (case 3): C and T nucleotides are both detected, representing a heterozygous mutation. (C) cDNA sequencing (case 3): no wildtype, only mutated mRNA is detected. (D) MSP result representing a lack of P53 promoter methylation (case 3). T, tumour sample; N, a corresponding normal tissue (blood); −, negative control; +, positive control; M, methylated; UM, unmethylated.
