Figure 3
From: Cyclopentenyl cytosine increases gemcitabine radiosensitisation in human pancreatic cancer cells
Treatment efficacy of preincubation with CPEC, a nonradiosensitising dose of dFdC and irradiation in human pancreatic carcinoma cells (Panc-1, Miapaca-2, BxPC-3) and NSCLC cells (SWp and its dFdC-resistant variant SWg). Cyclopentenyl cytosine enhanced the efficacy of dFdC combined with radiation with respect to cell proliferation in Panc-1 (A) and Miapaca-2, BxPC-3, SWp, but not in SWg cells (B). Clonogenic survival after radiation (C) is shown for Panc-1 and Miapaca-2 cells after correction of the toxicity of either treatment without irradiation (Panc-1: CPEC, 0.71±0.12; dFdC, 1.01±0.03; CPEC+dFdC, 0.40±0.03; Miapaca-2: CPEC, 1.00±0.06; dFdC, 0.52±0.20; CPEC+dFdC, 0.54±0.08). Induction of apoptosis (D) is shown for Panc-1, Miapaca-2 and BxPC-3. Representative examples (A, B and D) or means with standard errors are shown of at least three separate experiments (C, *P<0.05).
