Figure 4

Induction of NKD1 and FZD3 in cultured cells. (A) Lithium chloride induction of NKD1 mRNA in FHs74Int cells. Confluent cells were treated with lithium chloride and sampled over a 16-h time course. Expression of FZD3 and NKD1 was measured by real-time RT–PCR and the level relative to untreated control cells determined. The inset panel shows qualitative RT–PCR analysis of the induced cells at 0 and 16 h. (B) Wnt3a induction of NKD1 and FZD3 mRNA in HeLa cells. Confluent cells were treated with Wnt3a-conditioned medium and sampled over a 17-h time course. Expression of NKD1 and FZD3 was measured by real-time RT–PCR and the level relative to untreated control cells determined.