Table 1 Sequences of primers used in RT–PCR and real-time RT–PCR analysis
From: Reorganisation of Wnt-response pathways in colorectal tumorigenesis
Primers | TaqMan probe | |
|---|---|---|
Conventional RT–PCR | ||
NKD1 (NM_033119) | F, 5′-CGCCGGGATAGAAAACTACA-3′ | |
R, 5′-CTGGAGCTCTGAGACCTTGG-3′ | ||
FZD3 (NM_017412) | F, 5′-ACATCCACCCATGCTTCTTC-3′ | |
R, 5′-TCGTGACTGTTCATTGAGCC-3′ | ||
FZD4 (NM_012193) | F, 5′-CCAACATGGCTGTTGAAATG-3′ | |
R, 5′-GTCTCACTGCCTTTTCCAGG-3′ | ||
FZD6 (NM_003506) | F, 5′-ATGAGAGAGGTGAAAGCGGA-3′ | |
R, 5′-TTGGTTCTGAAGAACTGGGG-3′ | ||
FZD10 (NM_007197) | F, 5′-CCTCCAAGACTCTGCAGTCC-3′ | |
R, 5′-GACTGGGCAGGGATCTCATA-3′ | ||
Real-time PCR | ||
NKD1 (NM_033119) | F, 5′-TCGCCGGGATAGAAAACTACA-3′ | P, 5′-CCAATTTGGGCCTGGCTCCCC-3′ |
R, 5′-CAGTTCTGACTTCTGGGCCAC-3′ | ||
FZD3 (NM_017412) | F, 5′-CATGGAGATGTTTGGTGTTCCTT-3′ | P, 5′-TCTGGGAACCTACTGCATTCCATATCTTCAGG-3′ |
R, 5′-AAGTCGAGGATATGGCTCATCAC-3′ | ||
KRT8 (NM_002273) | F, 5′-GATCGCCACCTACAGGAAGCT-3′ | P, 5′-CCGGCTCTCCTCGCCCTCCA-3′ |
R, 5′-ACTCATGTTCTGCATCCCAGACT-3′ | ||
FZD6 (NM_003506) | F, 5′-ATCTGATGGGTCATTATGACCAGAGT-3′ | |
R, 5′- TTTGCGAGAGGAAGAAAATGCT-3′ | ||
