Figure 3

Western blot analysis of NGAL in a panel of pancreatic cancer cell lines. Protein lysates from 14 pancreatic cancer cell lines were resolved on a 15% SDS–PAGE gel and transferred to a PVDF membrane. After blocking with 5% BSA, the blot was hybridised with anti-NGAL antibodies (upper panel), stripped and re-probed with anti-β-actin (lower panel) to demonstrate loading of proteins in all lanes.