Figure 4 | British Journal of Cancer

Figure 4

From: Low expression of γ-glutamyl hydrolase mRNA in primary colorectal cancer with the CpG island methylator phenotype

Figure 4

Methylation-specific PCR analysis of the GGH promoter. Promoter methylation of GGH was analysed using unmethylated DNA-specific primer sets (U) and methylated DNA-specific primer sets (M). Representative results using 18 samples of CIMP+ and 20 randomly selected samples of CIMP− tumours are shown. Only one sample, a CIMP+ tumour, showed GGH promoter hypermethylation. Sperm DNA and fully methylated sperm DNA produced with SssI methylase were used for unmethylated control (UC) and methylated control (MC), respectively. DNA from the colon cancer cell line WiDr was also used as a positive control.

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