Figure 1 | British Journal of Cancer

Figure 1

From: SPARC promoter hypermethylation in colorectal cancers can be reversed by 5-Aza-2′deoxycytidine to increase SPARC expression and improve therapy response

Figure 1

Histological images of specimens of normal colon or colon cancer before (A-i, iv) and after (A-ii, iii, v, vi) laser capture microdissection (LCM); only colonic epithelium represented by images (iii) or (vi) were used for analysis. (B) Map of the CpG island in the promoter region at 5′ of the SPARC gene, including regions flanked by the bisulfite sequencing primers (P1–P5, arrows and sequences), numbered CG positions within the sequencing region (grey), exon1 (boxed). Brackets show regions amplified using methylation-specific primers MSP1 and MSP2. (C) Five sets of primers were used to sequence the regions shown in (B).

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