Figure 1

Manganese superoxide disumutase (MnSOD) expression in OSE and ovarian cancer cells. (A) Whole-cell lysates from different ovarian cell lines containing equal amounts of protein (50 μg) were loaded in each lane and analysed for expression of MnSOD by western blotting. β-Actin was also blotted as a protein-loading control. (B) O2•− level was measured by flow cytometry analysis using dihydroethidium. Solid histogram, CaOV-3 and SKOV-3 cells; open histogram, OVCAR-3.