Figure 10 | British Journal of Cancer

Figure 10

From: Chemosensitisation by manganese superoxide dismutase inhibition is caspase-9 dependent and involves extracellular signal-regulated kinase 1/2

Figure 10

Involvement of ROS production in the manganese superoxide disumutase (MnSOD) small-interfering RNA (siRNA)-mediated activation of caspase-9. SKOV-3 cells were transiently transfected with either the Ctrl or MnSOD siRNA for 48 h, and then pre-treated with N-acetyl cysteine (NAC, 5 mM) for 30 min followed by doxorubicin (DOX, 5 μ M) or paclitaxel (PTX, 0.1 μ M) addition for another 48 h. Cells were then analysed for expression of caspase-9 and caspase-8 proteins by western blotting using the respective antibodies. β-Actin serves as a protein-loading control.

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