Figure 2 | British Journal of Cancer

Figure 2

From: Chemosensitisation by manganese superoxide dismutase inhibition is caspase-9 dependent and involves extracellular signal-regulated kinase 1/2

Figure 2

Overexpression of manganese superoxide disumutase (MnSOD) reduces ROS production in OVCAR-3. (A) Whole-cell lysates of MnSOD stably transfected clones (M5, M17, and M24), parental (WT), and vector (Neo) control cells were harvested and analysed for expression of MnSOD by western blotting. β-Actin serves as a protein-loading control. (B) O2•− level was measured by flow cytometry analysis using dihydroethidium. Solid histogram, MnSOD-overexpressing clones; open histogram, Neo control. (C) MnSOD activity was measured. Values are mean±s.d. of three independent experiments. *P<0.05 vs WT and Neo control cells.

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