Figure 3 | British Journal of Cancer

Figure 3

From: Chemosensitisation by manganese superoxide dismutase inhibition is caspase-9 dependent and involves extracellular signal-regulated kinase 1/2

Figure 3

Overexpression of manganese superoxide disumutase (MnSOD) protects cells from doxorubicin (DOX)- and paclitaxel (PTX)-induced apoptosis in OVCAR-3. Manganese superoxide disumutase-overexpressing OVCAR-3 cells were exposed to DOX (5 μ M) or PTX (0.1 μ M) for 48 h and then harvested for (A) 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The absorbance of wells not exposed to treatments was arbitrarily set as 1, and cell growth after treatment was expressed as the fold changes compared with the control. Apoptosis was measured by (B) terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL) and (C) 4′,6-diamidino-2-phenylindole (DAPI) staining. Cells stained with DAPI exhibiting condensed, pyknotic, or fragmented nuclei were representative of apoptotic cells. Experiments were repeated three times, and data are shown as mean±s.d. *P<0.05 vs WT and Neo control cells.

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