Figure 4 | British Journal of Cancer

Figure 4

From: Chemosensitisation by manganese superoxide dismutase inhibition is caspase-9 dependent and involves extracellular signal-regulated kinase 1/2

Figure 4

Suppressing manganese superoxide disumutase (MnSOD) expression by small-interfering RNA (siRNA) increases ROS production in SKOV-3. (A) Cells transfected with non-specific control (Ctrl siRNA) or MnSOD siRNA were analysed for expression of MnSOD, Cu/Zn-SOD proteins by western blotting using the respective antibodies. β-Actin serves as a protein-loading control. (B) O2•− level was measured by flow cytometry analysis using dihydroethidium. Solid histogram, untransfected and Ctrl siRNA-transfected cells; open histogram, MnSOD siRNA-transfected cells. (C) MnSOD activity was measured. Values are mean±s.d. of three independent experiments. *P<0.05 vs WT and Neo control cells.

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