Figure 6
Involvement of ROS production in anti-cancer drug-induced apoptosis in ovarian cancer cells. SKOV-3 cells transfected with manganese superoxide disumutase (MnSOD) small-interfering RNA (siRNA) were pre-treated with glutathione (GSH, 5 mM) or N-acetyl cysteine (NAC, 5 mM) for 30 min, and then the cells were treated with doxorubicin (DOX, 5 μ M) or paclitaxel (PTX, 0.1 μ M) for another 48 h. (A) Apoptosis was measured by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL) assay and 4′,6-diamidino-2-phenylindole (DAPI) staining. Experiments were repeated three times, and data are shown as mean±s.d. *P<0.05 vs untreated control cells. (B) O2•− level of DOX- and PTX-treated cells was measured by flow cytometry analysis using dihydroethidium. Solid histogram, untreated cells; open histogram, DOX- and PTX-treated cells.
