Figure 7
Inhibition of manganese superoxide disumutase (MnSOD) does not sensitise chemotherapy-induced apoptosis in non-transformed OSE cells. IOSE-29 and IOSE-398 cells were transfected with non-specific control (Ctrl small-interfering RNA, siRNA) or MnSOD siRNA in the presence or absence of doxorubicin (DOX, 5 μ M) or paclitaxel (PTX, 0.1 μ M) for 48 h. (A) Expression of MnSOD protein was analysed by western blotting. β-Actin serves as a protein-loading control. (B) Apoptosis was assessed by the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL) assay. Experiments were repeated three times, and data are shown as mean±s.d. (C) O2•− level of DOX- and PTX-treated cells was measured by flow cytometry analysis using dihydroethidium. Solid histogram, untreated cells; open histogram, DOX- and PTX-treated cells.
