Figure 2
A negative correlation exists between extracellular cystine deprivation and expression of the xc− transporter. (A) Q-RT–PCR for expression of xCT or 4F2hc mRNA in MIA PaCa-2, PANC-1, and BxPC-3 cell lines incubated with various cystine concentrations (0.01, 0.1 or 1 mM) for 72 h. Data represent the mean±s.e.m. from three independent experiments. *P⩽0.05. (B) Western blot for expression of 4F2hc or tubulin in MIA PaCa-2, PANC-1, and BxPC-3 cell lines incubated with various cystine concentrations (0.01, 0.1 or 1 mM) for 72 h. Results are representative of two independent experiments.
