Figure 6
Overexpression of transfected xCT increases cystine uptake and increases GEM resistance. (A) RT–PCR for xCT and 4F2hc expression in MIA PaCa-2 cells transfected with pcDNA3.1 empty vector, pcDNA3.1-xCT plasmid, or pcDNA3.1-4F2hc plasmid. (B, C) Q-RT–PCR for xCT (B) or 4F2hc (C) expression in MIA PaCa-2 cells transfected with pcDNA3.1 empty vector, pcDNA3.1-xCT plasmid, or pcDNA3.1-4F2hc plasmid. *P<0.001. (D) [3H]-glutamate uptake assay in MIA PaCa-2 and PANC-1 cells transfected with pcDNA3.1 empty vector, pcDNA3.1-xCT plasmid, and/or pcDNA3.1-4F2hc plasmid. *P<0.01 compared with respective cell line pcDNA3.1 control. (E) Neutral red uptake assay for survival of untreated or GEM-treated MIA PaCa-2 cells transfected with pcDNA3.1 empty vector, pcDNA3.1-xCT plasmid, and/or pcDNA3.1-4F2hc plasmid. *P⩽0.05 compared with pcDNA3.1 control; #P⩽0.05 compared with pcDNA3.1 GEM treatment. All data represent the mean±s.e.m. from three independent experiments.
