Figure 5
Immunofluorescent localisation of claudin-4 in cell lines. Claudin-4 localisation was examined in the adherent cell lines ALVA41 (A), DU145 (B), LNCaP (C), PC3 (D), RWPE1 (E) and HeLa (F). Claudin-4 was detected with an anti-claudin-4 antibody and visualised with an anti-mouse IgG antibody conjugated to FITC (A–F). Nuclei of cells were counterstained with DAPI (G–L). Images were merged (M–R) to determine correct localisation. The metastatic prostate tumour cell lines ALVA41 (M), DU145 (N), LNCaP (O) and PC3 (P) revealed claudin-4 to be localised to the cell membrane between cell-to-cell contact sites. The normal prostate cell line RPWE1 (Q) and the negative control cell line HeLa (R) did not reveal any claudin-4 localisation.
