Table 3 Immunohistochemical evaluation of AMACR, PSMA and claudin-4 expression in prostate tissue sections

From: Identification of claudin-4 as a marker highly overexpressed in both primary and metastatic prostate cancer

 

Percentage and intensity of cells staining

 

<25%

25–50%

>50%

<25%

25–50%

>50%

<25%

25–50%

>50%

Normal prostate sections

AMACR (n=15)

PSMA (n=14)

Claudin-4 (n=29)

 Nil

12

  

1

  

3

  

 Low

3

  

3

6

2

3

12

4

 Moderate

   

1

1

  

5

2

 Strong

         

 Intense

         

BPH

AMACR (n=14)

PSMA (n=13)

Claudin-4 (n=19)

 Nil

12

  

2

  

1

  

 Low

1

1

 

1

2

4

 

1

3

 Moderate

   

1

2

1

1

6

5

 Strong

        

2

 Intense

         

HG-PIN (with no foci of PCa)

AMACR (n=11)

PSMA (n=11)

Claudin-4 (n=18)

 Nil

5

  

4

     

 Low

3

 

1

 

6

 

1

 

2

 Moderate

1

     

1

 

12

 Strong

 

1

  

1

 

1

1

 

 Intense

         

PCa

AMACR (n=21)

PSMA (n=21)

Claudin-4 (n=25)

 Nil

3

        

 Low

3

2

 

1

2

2

 

1

1

 Moderate

1

2

2

 

3

5

3

9

4

 Strong

 

1

6

1

1

6

3

2

1

 Intense

  

1

    

1

 

HG-PIN (with foci of PCa)

AMACR (n=19)

PSMA (n=18)

Claudin-4 (n=21)

 Nil

7

        

 Low

4

2

 

4

2

1

   

 Moderate

2

 

2

 

3

5

 

2

4

 Strong

1

 

1

  

3

1

8

6

 Intense

         

Benign glands (adjacent to PCa)

AMACR (n=21)

PSMA (n=21)

Claudin-4 (n=25)

 Nil

20

  

3

     

 Low

1

  

5

3

3

   

 Moderate

   

2

4

1

 

7

7

 Strong

      

2

6

3

 Intense

         

Metastatic sites

AMACR (n=18)

PSMA (n=16)

Claudin-4 (n=45)

 Nil

11

     

3

  

 Low

5

4

1

3

1

 

9

  

 Moderate

6

2

3

 

1

4

2

6

5

 Strong

  

6

4

2

1

 

3

13

 Intense

       

1

3

  1. Abbreviations: AMACR=α-methylacyl-CoA racemase; BPH=benign prostatic hyperplasia; HG-PIN=high-grade prostatic intraepithelial neoplasia; PCa=prostate cancer; PSMA=prostate-specific membrane antigen.
  2. Immunohistochemical staining for AMACR, PSMA and claudin-4 was performed on a number (n) of prostate sections. The percentage and the intensity of cells staining within each of the sections were determined by a uropathologist.