Figure 2

Migration of MSC to spheroids of pancreatic tumour cells. (A) Green-labeled spheroids (composed of 5 × 10² MIA-PaCa-2, 2.5 × 10² human primary fibroblasts, and 2.5 × 10² HUVEC) and red-labeled MSC were seeded in opposite edges of a well of a 24-well plate and covered with methocel/collagen solution as described in Materials and methods. (B) Cells were analysed using a fluorescence microscope (Olympus IX 70) with a red and green filter, 20-fold magnification and two-fold binning immediately after seeding. The focus was put to spheroids. Migration of red fluorescent MSC to green fluorescent spheroids was monitored by time-lapse photography during a period of 12 h and a picture was taken every 5 min. Representative data of one experiment out of three similar is shown.