Figure 4

Effects of DKK3-enriched media on EJ28 directional cell migration. Empty vector or DKK3 was overexpressed in COS7 cells and serum-free conditioned media enriched in DKK3 were used in the experiment. (A) Matrigel invasion assay. A total of 5 × 10⁴ cells were seeded in the upper chamber and incubated with either DKK3- or empty-vector-enriched media for 24 h. The number of cells invading through the membrane was counted; ^*P<0.005 between DKK3 and empty vector. (B) Photograph of Matrigel invasion assay. (C) Scratch test. Rate of closure of the scratch following incubation with conditioned media was measured at 24 and 48 h; ^**P<0.01 for DKK3 at 24 and 48 h vs empty vector at 24 and 48 h. (D) Photograph of the scratch test at 24 and 48 h. (E) Scratch test. Rate of closure of the scratch following incubation with aprotinin, BB94 and DKK3.