Figure 3
Expression of Mage-b in 4T1 primary tumours and metastases. The Mage-b-specific RT–PCR product of 632 bp was detected by southern blotting using DNA probe encoding Mage-b. β-actin (285 bp) was used to determine RNA quality. The lanes were loaded as follows: lane 1: normal breast tissue; lane 2: 4T1 tumour; lane 3: 4T1 tumour; lane 4: metastasis in peritoneal cavity (PC); lane 5: metastasis in PC; lane 6: metastasis liver; lane 7: metastasis liver; lane 8: metastasis spleen; lane 9: metastasis spleen; lane 10: metastasis kidney; lane 11: metastasis kidney; lane 12: metastasis diaphragm; lane 13: metastasis diaphragm.
