Figure 4
Strong effect of vaccination with LM-LLO-Mage-b/2nd on metastases but not on primary tumours in the 4T1 model. BALB/c mice were immunised with the various Listeria-based Mage-b vaccine strains and challenged with 4T1 tumour cells as outlined in Figure 1. Two weeks after tumour challenge, mice were euthanized and the number of metastases (A) and tumour size (B) was determined per mouse. LM-LLO-Mage-b/2nd was the most effective vaccine strain against 4T1 metastases, whereas none of the Listeria-based Mage-b vaccines had any inhibitory effect on tumour growth. These vaccine studies were repeated three times in independent experiments with the most effective vaccine strain, that is, the LM-LLO-Mage-b/2nd. Again, the number of metastases (C) and tumour size (D) was determined per mouse. Results were averaged and subjected to statistical analysis using Mann–Whitney test (n=5 mice per group in each experiment). The error bars represent the s.e.m. In panel C, each triangle represents the number of metastases per mouse, and the vertical bars represent the average number of metastases per mouse.
