Figure 6
From: Image-guided and passively tumour-targeted polymeric nanomedicines for radiochemotherapy
Characterisation of the gemcitabine (Gem)-containing HPMA copolymers. (A) and (B) Chemical structure of A-Gem (poly(HPMA)-AH-Gem) and B-Gem (poly(HPMA)-GFLG-Gem). (C and D) Release of gemcitabine from A-Gem and B-Gem at pH=7.4, at pH=6.0, and at pH=6.0 in the presence of the lysosomal cysteine protease cathepsin B (CB). Values are expressed relative to the total amount of drug conjugated to the copolymers, and they represent average±s.d. of three independent experiments. (E and F) Cytotoxicity of free gemcitabine, A-Gem, B-Gem and a drug-free control copolymer. Colony formation assays were performed using Dunning AT1 rat prostate carcinoma cells (E) and A2780 human ovarian carcinoma cells (F). Values represent average±s.d. (n=3).
