Figure 1
From: Genetic regulation of MUC1 alternative splicing in human tissues
(A) Diagram of the start of MUC1 showing the positions of the PCR primers in relation to rs4072037 (asterisked). The variable extension of exon 2 is shown in white. Tandem repeat domain is shown in dark grey. The positions of the single-sense primer and two antisense primers are shown with arrows. The antisense primer downstream of the TR domain is used for amplifying the components lacking the TR domain. (B) ALF Express trace of the RT–PCR from three individuals with different rs4072037 genotypes, using the primer in exon 1 in combination with the primer upstream of the TR domain. The positions of the four transcripts, a, b, c and d, as well as the 100 and 300 bp markers are indicated. (C) ALF Express traces from two AG heterozygous individuals, one of whom shows very little a transcript. Samples from foetal lung; complete traces are shown in Supplementary Figure 1.
