Figure 3
From: Hypoxia upregulates expression of human endosialin gene via hypoxia-inducible factor 2
Localisation of hypoxia-responsive regions in the endosialin promoter. (A) HeLa cells were transfected with 5′-progressively truncated endosialin promoter fragments in pGL3-basic luciferase vector (2 μg) and pRL-TK plasmid (100 ng). MutHRE construct contained mutations in two HREs (−1072/−1065 and −976/−969). The transfected cells were incubated for 24 h in normoxia or hypoxia. Luciferase activity was normalised against renilla activity. Empty pGL3-basic plasmid was used for control of the basal luciferase activity. (B) Scheme of the upstream regulatory region of the endosialin gene with indicated positions of predicted binding sites for transcription factors of the HIF family (HRE), ETS family (EBS) and SP1. Truncated promoter fragments are illustrated below with the 5′ termini scaled to scheme to facilitate allocation of the relevant cis elements.
