Figure 2
Knockdown of Net1 mRNA expression results in decreased AGS cell migration and invasion. (A) The effect of RNAi-mediated NET1 knockdown on AGS cell migration using a wound-healing assay in comparison with control AGS cells. Cells were treated with targeting or non-targeting siRNA for 48 h before the wound-healing assay. Cell migration was measured 24 h after the ‘wound’. Bar: 200 μm. (B) Graphical representation of the effect of NET1 siRNA on wound healing. (C) The in vitro migratory capacity of control and NET1 knockdown cells using trans-well migration chambers. Cells were treated with targeting or non-targeting siRNA for 48 h before the migration assay, equal numbers of cells (5 × 104) were added to the chambers to determine the effect of knockdown on migration over 24 h. (D) The in vitro invasive ability of control and NET1 knockdown cells using trans-well Matrigel invasion chambers. Cells were treated with targeting or non-targetting siRNA for 48 h before the migration assay, equal numbers of cells (5 × 104) were added to the trans-well chambers to determine the effect of knockdown on invasion over 24 h. (*P<0.05). Error bars represent standard deviation of triplicate experiments.
