Figure 3
Lysophosphatidic acid (LPA) drives NET1 expression in a dose–responsive manner and promotes AGS cell migration. (A) Expression of NET1 mRNA in AGS gastric cancer cells treated with various doses of LPA for 4 h, as detected by quantitative real-time PCR (*P<0.05). (B) Expression of NET1 protein in AGS gastric cancer cells treated with various doses of LPA for 4 h, as detected by western immunoblot, respectively. (C) The effect of 20 μ M LPA on AGS cell migration using a wound-healing assay in comparison with control AGS cells. Cells were grown to confluence, and immediately following the ‘wound’ fresh media was added with or without LPA. Cell migration was measured 12 h after the ‘wound’. Bar, 200 μm. (D) Graphical representation of the effect of LPA on wound healing. (*P<0.05). Error bars represent standard deviation of triplicate experiments.
