Figure 6
NET1 knockdown blocks the organisation of the actin cytoskeleton. Fluorescence microscopy of F-actin formation in (A) control AGS cells, (B) cells treated with LPA, (C) cells in which NET1 knockdown was achieved and (D) cells treated with LPA and NET1 siRNA. Cells were treated with siRNA for 48 h before treatment with or without LPA, 4 h after which the effect on actin polymerisation was assessed. F-actin was detected using TRITC-conjugated phalloidin (green). Nuclei were counter stained using propidium iodide (orange). Bar 40 μm.
