Figure 3
From: Involvement of Cyr61 in growth, migration, and metastasis of prostate cancer cells
Silencing of Cyr61 inhibited the migration of Du145 cells. (A) Wound-healing assay for either Du145/Ctrli #1 or Du145/Cyr61i #1 cells. (B) Boyden chamber assay for Du145/Ctrli #1 or Du145/Cyr61i #1 cells, and the cells migrated through the polycarbonate membrane were counted following H&E staining. Student's t-test (*P<0.01). (C) Boyden chamber assay for Du145 cells treated with either rabbit IgG (Fc) or the antibody against Cyr61 (anti-Cyr61) and the cells migrated through the polycarbonate membrane were counted following H&E staining. Student's t-test (*P<0.01). (D) Boyden chamber assay for LnCap cells treated with either BSA (1 μg ml−1) or the purified Cyr61-his protein. Cyr61 stimulates LnCap cell migration in a dosage-dependent manner.
