Figure 4

Silencing of Cyr61 changed the intensity and distribution of focal adhesion. Immunofluorescence of FAK and paxillin, two markers of focal adhesion, on either Du145/Ctrli #1 ((A) for FAK staining and (C) for paxillin staining) or Du145/Cyr61i #1 cells ((B) for FAK staining and (D) for paxillin staining). Scale Bar=10 μm. (E) Increased focal adhesion formation upon reduced Cyr61 expression. Box-and-whisker plots of paxillin-positive-stained points within Du145/Ctrli or Du145/Cyr61i plated on FN for 8 h (^***P<0.001; n=15 cells per point). (F) Increased FAK (pY397) and paxillin (pY118) levels upon reduced Cyr61 expression. Western blot detected protein level of total FAK and paxillin, together with FAK (pY397) and paxillin (pY118). β-Actin was used as a loading control.