Table 3 Modulation of gene expression as detected by microarray analysis and real-time PCR

From: A genome-wide expression analysis identifies a network of EpCAM-induced cell cycle regulators

 

A2C12

 

A549

Caco-2

 

Fold change

 

Fold change

Fold change

Gene

Microarray analysis a

Real-time PCR

Gene

Microarray analysis a

Real-time PCR

Microarray analysis a

Real-time PCR

Lats2

16.0

1.7

LATS2

9.9

2.5b

4.3

2.5b

Gadd45b

18.4

Inducedc

GADD45B

2.5

Inducedc

2.3

Inducedc

Mdm2

2.1

1.5b

MDM2

2.9

3.2b

Ncd

1.3

Pim1

13.9

Inducedc

PIM1

4.6

10.7b

2.1

2.9b

Trp53

−3.5

2.4b

TP53

Ncd

2.8b

Ncd

−1.5

Ccna2

2.6

2.4b

CCNA2

1.5

4.1b

Ncd

1.1

Ccnd2

−3.3

−3.2b

CCND2

Ncd

2.3

1.5

2.5b

  1. EpCAM=epithelial cell adhesion molecule.
  2. aIn the case of more than one probe set identifying modulated gene expression, the highest fold change value is shown. All values −2 or 2 are statistically significant for P<0.002. Fold changes between −2 and 2 have not been considered as significant according to the applied criteria (see Materials and Methods).
  3. bDifferences of gene expression between EpCAM antibody-treated and untreated cells were statistically significant (P<0.05).
  4. cFold change could not be calculated, as no expression was detectable in the non-treated control probe. Expression of the specific PCR product in the antibody-treated probes was shown by agarose gel electrophoresis (Supplementary Figure S1).
  5. dNc: no change.