Figure 5

IP6 and proteasome inhibitors present an enhanced effect on mitochondrial depolarisation. (A) PC3 cells were pre-treated with 50 μg ml⁻¹ cycloheximide (CHX), 20 μ M MG-132 (MG) or control ethanol and the percentage of cells presenting mitochondrial depolarisation according to JC-1 staining was assessed by FACS after 0, 2, 4 and 8 h of treatment with 2 mM IP6 (or vehicle). (B) PC3 cells were pre-treated with 20 μ M MG-132, 10 μ M ALLN or DMSO before the addition of 2 mM IP6 (or control). Eight hours later, the percentage of cells presenting mitochondrial depolarisation according to JC-1 staining was assessed by FACS. (C) PC3 cells were pre-treated with 20 μ M MG-132, 50 μg ml⁻¹ cycloheximide or a combination of MG-132 and cycloheximide or control ethanol before addition of 2 mM IP6 (or vehicle). Eight hours later, the percentage of cells presenting mitochondrial depolarisation according to JC-1 staining was assessed by FACS. In (A–C), data were normalised according to the percentage of cells exhibiting mitochondrial depolarisation according to the JC-1 staining observed in vehicle-treated controls. Data represent an average of two to three independent experiments. All error bars represent the standard error.