Figure 2

(A) Tissue section of enucleation specimen after dissection of four tumour areas (1–4) and one normal tissue area (N). (H&E, magnification × 1.25). (B) Silver-stained polyacrylamide gel showing a single band at 200 bp in the four corresponding tumour samples and in the normal tissue sample indicating lack of the T1799A point mutation in this case. (C) Tissue section of local resection specimen after dissection of four tumour areas (1–4). (H&E, magnification × 2). (D) Silver-stained polyacrylamide gel showing an additional band at 100 nt in samples 2 and 4 indicating the presence of the mutation in these samples. There is an additional band between 200 and 100 nt representing a PCR artefact. Samples 1 and 3 show only one band at 200 nt indicating lack of the mutation.