Figure 1

Apoptosis induction by MegaFasL in GIST cell lines. (A) Analysis of Fas membrane expression by flow cytometry. The thin grey line represents the IgG control and thick black line reflects the anti-Fas antibody. HeLa was used as a positive control. (B) Representative images of acridine orange staining of GIST882 and GIST48 with or without treatment with 50 ng ml⁻¹ MegaFasL for 6 h. Nuclear fragmentation is seen in cells treated with MegaFasL. (C) GIST cell lines, and HeLa, were incubated with different concentrations of MegaFasL for 6 h. Apoptosis was quantified with acridine orange staining. Data represent the mean±s.d. of three independent experiments. (D) Expression of caspases 8, 6, 3, and cleaved caspase 3 after treatment of GIST882 and GIST48 cells with 50 ng ml⁻¹ MegaFasL for 6 h, analysed by western blot. Immunoblotting of actin was used as a loading control.