Figure 2 | British Journal of Cancer

Figure 2

From: Blockade of SDF-1/CXCR4 signalling inhibits pancreatic cancer progression in vitro via inactivation of canonical Wnt pathway

Figure 2

Establishment of stable CXCR4 knockdown. (A) QT–PCR analysis of CXCR4 expression after the transfection of different CXCR4 shRNA expression vectors: pRNAT-M1 (target position: 301), pRNAT-M2 (target position: 1093), pRNAT-MN (negative control) and Miapaca-2, *P<0.05 compared with pRNAT-MN cells; (B) western blot with CXCR4 antibody for different transfected pancreatic cancer cells. GAPDH was detected as a loading control; (C) Western blot showed the different expressions of CXCR4, phosphorylated CXCR4 on Serine 339 (P339-CXCR4) on transfected cells. GAPDH was detected as a loading control.

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