Figure 4

The influence of CXCR4 knockdown on Wnt target gene and invasion-related genes. (A) β-Catenin/Tcf transcription reporter assay. Normalised with control reporter plasmid, the relative luciferase activity was demonstrated. ^*P<0.05 compared with pRNAT-MN cells; (B) QT–PCR analysis to examine the CTNNB1 (β-catenin), UPA, SLUG, CD44, MYC(c-MYC), Vim (Vimentin), CCND1 (cyclin D1) gene expression by the $2^{\text{-ΔΔ}{C}_{\text{t}}}$ method. ^*P<0.05 compared with pRNAT-MN cells; (C) western blot analysis to detect MMP-9 and Vimentin protein expression. GAPDH was used as a loading control.