Figure 2

(A) Effect of 2DG on the proliferation of LNCaP and MCF-7 cells. Cells were cultured in 96-well plates and treated with 2DG (0.005–50 mM) for 96 h when their effects on proliferation were measured using a microtitre plate assay. Effect of 2DG and hypoxia on ATP levels in MCF-7 (B) and LNCaP cells (C). Cells were cultured in 96-well plates under normoxia and treated with 2DG (3 mM) for 72 h when their effects on ATP were measured using the microtitre, ATPlite plate assay. Results are expressed as percent of untreated. At this dose of 2DG, no significant inhibition of cell proliferation occurred (data not shown).