Figure 3

Effects of 2DG and STX140 on the proliferation of MCF-7 (A) and LNCaP (B) cells. Cells were cultured in 96-well plates, under normoxia or hypoxia, and treated with 2DG (8 mM) and/or STX140 (0.1–1 μ M) for 72 h when their effects on proliferation were measured by using a microtitre plate assay. Results are expressed as percent of proliferation of untreated cells in normoxia. In both cell lines 2DG alone was significantly (P<0.001) more potent in hypoxia vs normoxia. There was no significant benefit of 2DG+STX140 in MCF-7 cells. In LNCaP cells under normoxia, 2DG+0.1 μ M STX140 gave significantly greater inhibition than 2DG alone (^*P<0.05) and 0.1 μ M STX140 alone (P<0.001). Under hypoxia, 2DG+0.5 μ M STX140 and 2DG+1.0 μ M STX140 gave significantly greater inhibition than 2DG alone (^*P<0.05 and ^**P<0.01) and 0.5 and 1.0 μ M STX140 alone (P<0.001) (ns=not significant vs untreated normoxia).