Figure 4

Effects of 2DG, STX140 and hypoxia on cell cycle and apoptosis in MCF-7 (A) and LNCaP (B) cells. For cell cycle analysis the cells were fixed, treated with RNase, stained with propidium iodide and analysed using a flow cytometer (FACScan, Becton Dickinson, Cowley, UK). For quantification of apoptosis the cells were re-suspended in binding buffer and then stained with fluorescein-conjugated Annexin V antibody and propidium iodide before flow cytometric analysis. Apoptotic cells are defined as cells positive for Annexin V and negative for propidium iodide. All treatments lasted for 48 h (^ΔΔΔP<0.001 vs normoxia control; ^□P<0.05 vs normoxia control, hypoxia control and 2DG alone in normoxia; ^▪▪P<0.01 vs normoxia control; ⁺⁺⁺P<0.001 vs normoxia STX140; ^**P<0.01 vs normoxia and hypoxia control; ^••P<0.01 vs STX140 in hypoxia 2DG combined with STX140 under normoxia; ^♦♦♦P<0.001 vs normoxia control; and ns=not significant).