Figure 3
Jun N-terminal kinase (JNK) activation is involved in γ-T3-induced apoptosis. (A) Cell viability, after incubation with γ-T3 and JNK inhibitor (SP600125) for 24-h, was examined by an MTT assay. Note that the addition of JNK inhibitor alleviates the cytotoxicity of γ-T3 in PC-3, suggesting that JNK mediates the antiproliferation effect of γ-T3. (B) JNK activity after 24-h dose-dependent and IC50 time-dependent γ-T3 treatment (in μ M and hours respectively) and was found to be elevated by measuring the phosphorylation levels of MKK4, SAPK/JNK, c-jun and ATF-2. Thus, confirming the involvement of JNK in γ-T3 anticancer property.
