Figure 1

Laser-capture microdissection pressure catapulting (LMPC) and expression profiling of basal and luminal prostate epithelial cells. (A) Snap-frozen BPH tissue was rapid immunostained for basal cell marker K14, with nuclei counter stained with haematoxylin (a). Basal and luminal epithelial cells were laser captured sequentially; luminal cells were selected (b), then captured (c), followed by selection (d) and capture (e) of K14-positive basal cells. Extracted RNA from five patients was used for expression profiling by cDNA microarray. Differentially expressed genes were identified through ANOVA (P=0.05). Genes were entered into hierarchical cluster analysis represented here by a dendrogram. (B) Semiquantitative RT–PCR of amplified RNA from three patient samples (1, 2 and 4) confirming differential basal (B) and luminal (L) expression of SNAP25, TEAD1 and integrin αV. Luminal expression of SNAP25 was confirmed in three out of three patients; basal expression of TEAD1 was confirmed in two out of three patients and basal expression of integrin αV was confirmed in one out of three patients.