Figure 2 | British Journal of Cancer

Figure 2

From: Simultaneous blockade of AP-1 and phosphatidylinositol 3-kinase pathway in non-small cell lung cancer cells

Figure 2

Effects on PI3K/Akt pathway activation of LY294002, TAM67 and their combination in H1299 cells. (A) H1299-Tet-on-TAM67 clone cells (TAM67 no. 8) and (B) H1299-Tet-on-GFP clone cells (GFP 1). Western blots of cell lysates incubated with antibodies against phospho-Akt (S473) and Akt in cells treated with LY294002, the induction of TAM67 and their combination. Representative radiographs of three independent experiments. Standardisation was with actin measured in the same blots. Comparison of protein expression levels among the various conditions is based on the ratio of expression of a protein in each condition to that in non-treated one (set equal to 1). NIH image 1.62 software was used to densitise and quantify the amount of the bands. The data show the average value of three independent experiments with error bars representing s.d. PC, positive control; NC, negative control.

Back to article page