Figure 5

(A and B) Activation of STAT3 in response to IL-6 in OVCA 433 cells. Expression of P-STAT3 in response to IL-6 in OVCA 433 cell line. OVCA 433 cells were incubated in normal growth medium (control) or medium treated with IL-6 (20 ng ml⁻¹) for 1 h. Western blot analysis was performed as described above for P-STAT3, P-ERK1/2 followed by re-probing first with T-STAT3, T-ERK1/2, respectively, followed by β-actin. (C) Effect of neutralising IL-6R antibody on the EGF or IL-6-induced migration in OVCA433 cells. Cells were cultured on glass coverslips in either normal growth medium (control) or EGF-treated medium (10 ng ml⁻¹) or IL-6-treated medium (20 ng ml⁻¹) in the presence and absence of IL-6R antibody (20 μg ml⁻¹). Using scratch tests over 14 h, cells were analysed for the extent of wound closure as described in the Materials and Methods. ^*P<0.05, compared to control untreated cells, ^§compared to cells treated with EGF or IL-6, respectively. (D) Effect of neutralising IL-6R antibody on IL-6 production in OVCA 433 cells. IL-6 production in the serum-free medium in the presence of +/−EGF and +/− anti-IL-6R or control serum was measured by sandwich ELISA assay as described in the Material and Methods. ^*P<0.05, compared to control untreated cells, ^§compared to cells treated with EGF.